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Automated Terpenes Analysis using GC-VUV Analyzer

Terpenes contribute greatly to the senses of smell and taste and thus are integral to industries like food and fragrance, cosmetics, and pharmaceuticals, among many others.

Vacuum UV (VUV) spectroscopy can spectrally distinguish isomers and deconvolve coeluting peaks, which promotes qualitative and quantitative accuracy and allows for significant chromatographic compression. Here an intra- and interdetector comparison is presented using three VUV methods (“slow,” “fast,” and “faster”) and two MS methods (“slow” and “fast”).

Tarpens VUV
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Chromatograms of VUV Methods

However, because VUV spectroscopy can account for the entire 3-dimensional electronic structure of the molecule, the absorbance spectra of structural isomers can be distinguished visually.

Utilizing these unique absorbance spectra, along with the fact that our detector technology is at ambient pressure and thus not flow limited, allows us to deliberately compress our chromatography by increasing the GC column flow and oven temperature program rate. We ran a terpenes standard mix in which 21 mono- and sesquiterpenes eluted in less than 9 minutes.

Advantages of VUV Spectroscopy and Terpenes solution Package

  • VUV Analyze provides a new tool for automating flavour and fragrance analysis.
  • Rich 3D data set: absorption vs. wavelength vs. retention time
  • Spectroscopic deconvolution simplifies chromatographic separation challenges.
  • Enables chromatographic compression for higher analytical productivity.
  • Flavors & Fragrance isomers have distinct spectra and can be unambiguously differentiated.
  • Easy to understand analysis by Beer-Lambert Law (same principle used in UV-Vis spectroscopy)
  • A fast GC–VUV and static headspace method to identify and quantitate terpenes. Chromatographic compression and shorter GC run times.
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