Agilent Seahorse XF Pro Analyzer

Agilent Seahorse XF Pro Analyzers measure the oxygen consumption (OCR) and extracellular acidification rate (ECAR/PER) of live cells in a 96-well format. The XF Pro Analyzer features better OCR precision at low rates, verified instrument performance and repeatability specifications, optimized temperature control, and is automation enabled.

OCR and ECAR or PER are key indicators of mitochondrial respiration and glycolysis as well as ATP production rate. Together, these measurements provide a systems-level view of cellular metabolic function in cultured cells and ex vivo samples.

Advanced data analytics are now available with Agilent Seahorse Analytics. Agilent Seahorse XF Pro software features new tools for easier plate set up, plate layout importation, heat map view, and dose response curve view. A new data quality view automatically flags erroneous data and makes it easier to reject outlier data points.

Agilent Seahorse XF Pro
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Key Applications

  • Optimization of experimental conditions before scaling up
  • Rare, sorted, and/or primary immune cells
  • Quiescent and low-respiring cell types
  • Cell lines and ex-vivo samples
  • Phenotypic screening
  • Testing many conditions at one time
  • Dose-response studies
  • Spheroids
  • Islets
  • Small-model organisms

Discover the Power of Cellular Metabolism

The Agilent Seahorse XF platform measures the two main metabolic pathways, mitochondrial respiration and glycolysis, for live cells in real time, to provide functional kinetic measurements of cellular bioenergetics. Discover how bioenergetic parameters can provide powerful insights and can serve as an indicator for disease models, critical cell processes, and therapeutic discoveries.

Immune Metabolism

Immune cell processes including activation, proliferation, and memory development are all driven by metabolic reprogramming, which can be tuned to enhance performance and control immune cell outcomes. Discover insights into immune cell processes including activation, proliferation, and memory development through functional real-time metabolic measurements.

Cancer Metabolism

Metabolism is a critical driver of cancer malignancy, with cancer cell proliferation commonly requiring an upregulation or “metabolic switch” towards a more glycolytic phenotype to fuel the rapid energy requirements and generate building blocks for cancer cell growth. Reveal cancer metabolic liabilities through functional bioenergetic analysis of live cells in real time for a deeper understanding of cancer cell biology.

Complete Platform for Live-Cell Metabolic Assays

Comprising instruments, assay kits, software, and consumables, Agilent seahorse XF platforms are designed to support you from sample to insights.

Agilent offers a complete workflow for live cell metabolic analysis including cell metabolism analyzers, liquid handling automation, imagine plate readers, and high quality consumables. All work together to help you achieve consistent, reproducible data.

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