17 Mycotoxins in One Go

Mycotoxins are toxins produced by certain fungi which grows on food items like Cereals, Dried fruits, Nuts, Spices etc.; stored in warm and humid conditions. These toxins are also found on feed thus it poses a serious health threat to both humans and livestock. Although multiple Mycotoxins have been identified, routinely around 12 Mycotoxins are regulated. Liquid chromatography Mass spectrometer is the popular instrument of choice owing to its better selectivity and sensitivity. Sample clean-up is a crucial part of the entire workflow as it enhances sensitivity with less matrix interference.

The CrossTOX is a QuEChERS column which has a simplified one step procedure for clean-up after extraction in solvent.

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  • One column for wide range of matrices.
  • Less dependency on internal standards owing to simplified workflow and excellent recovery.
  • Less cleaning and maintenance of Mass spectrometer due to the high purity of the extracts.
  • As the sample is passed through the column no separate filtration is required.

Mycotoxins tested with excellent recovery in variety of matrices

  • Aflatoxins B1,B2,G1 & G2
  • T-2
  • 15-Acetyl-DON
  • Ochratoxin A
  • HT-2
  • DON-3-Glc
  • Zearalenone
  • Nivalenol
  • Sterigmatocysine
  • Deoxynivalenol
  • 3-Acetyl-DON
  • Citrinin
  • Fuminosins B1, B2


With FREESTYLE SPE robotic system the clean-up process can be automated. The cleaned extracts can be collected in HPLC vial or can be injected directly to LC-MS/MS system.

FREESTYLE SPE robotic system

Multi-Mycotoxin Analysis CrossTOX

Application Note "Sample Clean-up and Matrix Removal"

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