GBA Group join hands with Cytosurge AG

For multiplex genome editing and fast cell line development.

The discovery of CRISPR-Cas9 for targeted gene engineering has revolutionized life sciences. Regardless of its broad introduction across many disciplines in biology, several challenges persist in editing cell lines, particularly for sensitive cells, or when multiple edits are required.

FluidFM offers a unique in-vitro gene editing supporting tool to improve the efficiency and applicability of CRISPR across a variety of cell types and for cell line development. 

Profiling to unveil cellular dynamics.

In the intricate evolution of cellular pathways, cells constantly adapt to their intrinsic programs and external triggers. Therefore, it is imperative to unravel the intricacies of these dynamic transformations and the mechanisms that drive them. Such insight is pivotal for gaining a profound comprehension of cellular processes, both in states of physiological and pathological conditions.

Sequencing to tackle cellular heterogeneity.

In the study of cellular dynamics, the inherent heterogeneity among cells necessitates a meticulous examination of their transitions at the individual cell level. Throughout the years, various methods, from live-cell imaging to molecular recording approaches, were employed to unravel the intricacies of cellular behavior.

Profiling and inferring cellular dynamics.

To complement those snapshots of cellular states, scientists worked on computational methods to infer cellular dynamics.

Sequencing while keeping cells alive: Live-seq

This approach enables time-resolved transcriptomics by extracting cytoplasmic biopsies from individual cells while keeping them alive, and thereby enabling repeated sampling from the same cell.

Traditionally, in Single-Cell Force Spectroscopy (SCFS) assays, the object of interest is glued to an AFM cantilever resulting in complex handling and low throughput. The FluidFM systems solve this issue by reversibly immobilizing a cell to a FluidFM probe via suction, and subsequent release with a pressure pulse or brief washing.