The future of functional metabolic analysis is here

The Agilent Seahorse XF Flex analyzer measures and reports the oxygen consumption rate (OCR), proton efflux rate (PER) or extracellular acidification rate (ECAR), as well as ATP production rates in a 24-well format. It offers optimized workflows and dedicated consumables to enable real-time metabolic analysis with 3D study models, such as organoids and tissue materials. It also provides wide signal dynamic range through the use of dedicated consumables.

The XF Flex analyzer is also equipped with advanced software and advanced data analytics available in the Agilent Seahorse Analytics software. These features greatly simplify the entire XF assay experience, from assay design to data QC and interpretation.

Seahorse XF Flex Analyzer
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Features

  • A flexible 24-well system that allows real-time metabolic analysis with improved sensitivity and consistency, using two-dimensional (2D) and three-dimensional (3D) study models
  • Multiple parameters reported from each assay well, including OCR, PER or ECAR, and ATP production rates
  • Tailored workflows and dedicated consumables for working with organoids and tissue materials
  • Wide detection range and great for high respiration study models
  • Four-port injection system with automated mixing feature for assessing immediate cellular, tissue organoid responses to substrates, inhibitors, and other compounds in real time
  • Verified instrument performance to ensure intraplate and interplate data consistency
  • Broad operation environment temperature of 4 to 30 °C, allowing the analyzer to maintain internal assay temperature of 16 to 42 °C
  • Intuitive, user-guided software for easy assay protocol creation
  • Automated data quality check in data analysis software to flag abnormal assay behavior
  • Data files compatible with advanced data analysis features in the Seahorse Analytics which is a secure web-based data analysis tool
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How It Works

Monitors bioenergetics of live cells, in real time, in a microplate

The two major pathways to produce energy, mitochondrial respiration and glycolysis, involve cellular consumption of oxygen and efflux of protons, respectively. Seahorse XF technology uses label-free sensors to detect extracellular changes in these analytes and report rates of cellular respiration, glycolysis, and ATP production. It can be used to interrogate metabolic changes in cultured cells, suspension cells, as well as three-dimensional materials such as tissue, organoids and/or multiple cell clusters.

Forms a microchamber and calculates rates of extracellular flux in minutes

The instrument lowers the cartridge probes into the assay wells. The sensors are positioned at a defined distance above the well bottoms, forming very small transient microchambers that are critical to the high sensitivity uniquely offered by Seahorse XF technology. As the oxygen and pH levels change, the instrument records the changes in the sensors. Measurements are typically made for three minutes and rates are calculated automatically. Upon completion of each measurement the probes are raised, allowing the extracellular medium to come back to baseline conditions.

Injects up to 4 compounds to test responses or interrogate biology in real time

The sensor cartridge also contains ports (4 per well) to enable injection of modulators into the cell wells during the assay. When specified by the instrument protocol, the system injects compound "A" into the assay wells and performs a gentle mixing step to ensure distribution of the compound throughout the assay medium. All wells are processed in this manner simultaneously. Subsequent measurement cycles, any additional injections specified by the protocol, and rate calculations are performed automatically.

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Applications

Discover the Power of Cellular Metabolism

The Agilent Seahorse XF platform measures the two main metabolic pathways, mitochondrial respiration and glycolysis, for live cells in real time, to provide functional kinetic measurements of cellular bioenergetics. Discover how bioenergetic parameters can provide powerful insights and can serve as an indicator for disease models, critical cell processes, and therapeutic discoveries.

Immune Metabolism

Immune cell processes including activation, proliferation, and memory development are all driven by metabolic reprogramming, which can be tuned to enhance performance and control immune cell outcomes. Discover insights into immune cell processes including activation, proliferation, and memory development through functional real-time metabolic measurements.

Cancer Metabolism

Metabolism is a critical driver of cancer malignancy, with cancer cell proliferation commonly requiring an upregulation or “metabolic switch” towards a more glycolytic phenotype to fuel the rapid energy requirements and generate building blocks for cancer cell growth. Reveal cancer metabolic liabilities through functional bioenergetic analysis of live cells in real time for a deeper understanding of cancer cell biology.

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Seahorse XF Flex analyzer and 3D applications

The Agilent Seahorse XF Flex analyzer takes metabolic research beyond the limits of 2D, unlocking real-time insights into how metabolism behaves in 3D models like tissues and organoids.
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Specifications

Analyzer ApplicationCellular Energy Metabolism Studies
Phenotyping
2D Cultures
Tissue Models
Organoids
Small Model Organisms

Assay Running Volume600-1000 µL/well

Assay Wells22

CommunicationTCP/IP
USB
Wireless

Controller DescriptionIndependent workstation with Windows 10 (64-bit) OS and touch-screen display, cable-connected to the analyzer, which allows full assay design, instrument control, and analysis capability

Dimensions (WxDxH)38.74 cm x 47.72 cm x 58.42 cm

Memory16 GB

Operating Environment Relative Humidity20-80 %

Operating Environment Temperature4-30 °C

Power Requirements100-240 VAC, 9 A, 50/60 Hz

Sample Requirements8000-500000 cells/well

Sample Temperature16-42 °C

Software IncludedWindows 11 Enterprise
Flex Controller
Wave Pro

Weight21.8 kg

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