Nicoya Alto for Biologics Research

Fully automated analysis for biologics

Whether it’s minimizing your sample preparation steps or using past learnings to optimize your results, Alto’s intuitive and automated workflows will take the guesswork out of SPR and guide you from sample to answer. Alto helps in developing automated characterization and binding capabilities, workflows for quantitating active species in solution, and flexible cartridge layouts for multi-functional assays, making Alto the perfect companion to any discovery workflow.

New cartridge configurations

SPR assays are not a one-size-fits-all, so your consumables shouldn’t be either. As the heart of your SPR experiment, it’s critical that each cartridge uniquely maps to your assay to maximize your throughput and allow for analysis of the most complex assays. That is why we’re developing cartridges with additional well layouts and lower-throughput options to offer more flexibility and ensure you get the most out of each consumable.

Reagent kits for sensor preparation

The key to success behind every SPR assay is the use of the optimal reagents for your interaction type. To further simplify your workflow and increase consistency across your experiments, we’re designing a range of reagent kits for common application types that will easily integrate with your Alto cartridges.

Comparison of Alto to Conventional SPR

A capture multi-cycle kinetic investigation of HuCAL Fab-MH binding to eGFP performed in parallel with Alto and a conventional SPR instrument revealed :

• Alto’s precise ligand recapture with mean <10% CV
• Highly comparable kinetic and affinity results
• No manual dilutions, degassing or data processing with Alto
• <30 min of operator time with Alto, instead of >90 min

Application

• Kinetics / Affinity Characterization
• Quantitation
• Screening
• Epitope Mapping / Binning
• Nanoparticle Characterization