NovoCyte Penteon Flow Cytometer Systems 5 Lasers

The NovoCyte Penteon flow cytometer builds on its successful predecessor, the NovoCyte, and provides an expanded set of capabilities that accommodate today’s high-end and increasingly sophisticated multi-color flow cytometry assays. You now have the flexibility to choose from up to 30 fluorescence channels utilizing up to 5 lasers with up to 30 independent detectors. 

NovoCyte for Core Lab Facility
Request Info/Quote


  • Expanded flexibility with 30-fluorescence channel options using 5 lasers
  • High sensitivity and resolution
  • Intuitive and powerful software for data acquisition, analysis, and reporting
  • Smart-design functionalities and walk-away operation to simplify your workflow
  • Automation-ready capability for high throughput needs
  • Wide, 7-log dynamic range eliminates the need for routine detector adjustments

Novo Sampler Q

NovoSampler Q can be integrated into different laboratory automation platforms and efficiently processes both FACS tubes (using a 40-tube rack) and 24-, 48-, 96-, and 384-well plates. The intuitive and industry leading NovoExpress software has been further advanced, providing an exceptional user experience in data acquisition, analysis and reporting. For Research Use Only. Not for use in diagnostic procedures.

Use of flow cytometry has become routine across scientific disciplines, encompassing both the basic and clinical research spaces. It has proven to be a powerful tool to analyze different subpopulations of immune cells and gather a comprehensive overview of the immune system.

Due to great advancements within the field, newer flow cytometers can support multiple colors, enabling identification of a variety of cells simultaneously. In this application note, a 30-color immunophenotyping panel was designed for the five-laser Agilent NovoCyte Penteon flow cytometer to see the distribution of immune cells in the lung, spleen, and mediastinal lymph nodes of mice infected with influenza.

Key Highlights

  • The Agilent NovoCyte Penteon flow cytometer enables researchers to study multiple important parameters in one acquisition—a great asset in immunophenotyping.
  • With five lasers and 30 detectors, it is possible to extract more information from a single tube, saving precious biological material.
  • Absolute count provides even more information about the sample.
  • In this application note, the same gating strategy was applied on three lymphoid organs, permitting comparison of T cell distribution and allowing insight into the immune system during influenza infection.
  • The high detection capabilities of the NovoCyte Penteon also allowed identification of other immune cell subsets in the lung, spleen, and lymph nodes in the same experiment.
Request Info/Quote


  • Apoptosis Assay
  • Immunophenotyping
  • Cell Proliferation
  • Cytokine Detection
  • Intracellular Protein Detection
  • Cell Cycle Analysis

Application Note

Cell Analysis

Everything You're Looking For In Your Flow Cytometer

Browse by